Vesicle detection

Extracellular vesicles (EVs) are small particles surrounded by a lipid bilayer. Most cells are capable of secreting EVs. They carry cargos between cells and play roles in diverse processing from embryo development to injury response, and from angiogenesis to disease progression. The typical size of EVs is in the range from 50-1000 nm, and TEM is the most convenient instrument for their visualization. When analyzed by conventional TEM with heavy metal staining, most EVs exhibit a characteristic cup-shaped morphology. Here you can automatically detect and measure EVs in your images

Result image

Detection is based on the Mask R-CNN. Each particle is identified, labeled, and measured. The green lines show the detected edges of the particles, and the red lines show the ellipses used for approximation.

Result table

The measurement results are stored in a .csv file with the following parameters:

Dataset and code available here

Upload up to 10 images for detection
Supported 8 and 16 bit images in formats: png, jpg, jpeg, tif, tiff

If you use ScanEV for your research, please cite the article "ScanEV – A neural network-based tool for the automated detection of extracellular vesicles in TEM images" by Igor Nikishin, Ruslan Dulimov, Gleb Skryabin, Sergey Galetsky, Elena Tchevkina, and Dmitry Bagrov // Micron, Vol. 145, 103044 (2021), doi: 10.1016/j.micron.2021.103044

This work was supported by RFBR, project №19-34-90148.